Incorporation of influenza virus M-protein into liposomes

Incorporation of Lysozyme into Liposomes

To define a model for enzyme latency in vitro, the capture was studied of egg white lysozyme by liposomes (phospholipid spherules in the smectic mesophase). Gel filtration resolved liposomes formed in the absence of enzyme from free enzyme when both were chromatographed immediately after mixing. In contrast, if liposomes were swollen in the presence of lysozyme, subsequent chromatography disclo...

Selective incorporation of influenza virus RNA segments into virions.

The genome of influenza A virus is comprised of eight viral RNA (vRNA) segments. Although the products of all eight vRNA segments must be present for viral replication, little is known about the mechanism(s) responsible for incorporation of these segments into virions. Two models have been proposed for the generation of infectious virions containing eight vRNA segments. The random-incorporation...

Preparation of Influenza Virosomes by Post- Insertion of Influenza Virus Spike Proteins into Preformed Liposomes

Over Expression of Influenza Virus M2 Protein in Prokaryotic System

Background and Aims: Influenza A virus of Orthomyxoviridae family is able to create pandemic influenza. Vaccination is the most effective way to prevent influenza virus infection. Matrix protein 2 (M2) is a homotetramer ion channel with 97 amino acids length and highly conserved among influenza viruses and is considered for development of a universal influenza vaccine. Materials and Methods: We...

Hemolysis by liposomes containing influenza virus hemagglutinins.

Liposomes containing influenza virus hemagglutinin were reassembled from envelopes solubilized with Nonidet P-40 and were shown to induce hemolysis and cell fusion at low pH.